藜麦提取物体外抗氧化活性研究

相启森; 张丽华; 姜亭亭; 张文杰; 李银丽; 申瑞玲

doi:10.13386/j.issn1002-0306.2016.02.007

藜麦提取物体外抗氧化活性研究

Determination of the in vitro antioxidant capacity of quinoa seeds extracts

摘要

摘要: 以产自山西的藜麦为研究对象,以对DPPH·和ABTS+·的清除能力、铁还原力等为指标,评价了其提取物的体外抗氧化活性。结果表明,藜麦提取物对DPPH·和ABTS+·均有较强的清除能力,其IC₅₀分别为（43.13±2.41）mg/m L和（27.91±1.46）mg/m L;FRAP法测得藜麦抗氧化能力为（1.90±0.10）mg Fe SO₄/g;此外,藜麦提取物能够有效抑制自由基引发的亚油酸过氧化和牛血清白蛋白氧化降解。藜麦提取物具有较强的体外抗氧化活性,为其体内抗氧化活性的研究奠定了基础。

Abstract: The aim of this study was to investigate the in vitro antioxidant activities of extract from quinoa seeds collected from Shanxi using various methods,including DPPH· and ABTS+· scavenging assays and ferric reducing antioxidant power(FRAP) assay. The results showed that the quinoa extract had strong scavenging activities for DPPH· and ABTS+· radicals. The radical scavenging IC_(50) values of quinoa extract were(43.13±2.41) mg/m L and(27.91±1.46) mg/m L for DPPH· and ABTS+· radicals,respectively. The FRAP value of quinoa was(1.90±0.10) mg Fe SO_4/g. The quinoa extract also significantly inhibited free radical-induced peroxidation of linoleic acid and oxidative degradation of bovine serum albumin. The present study demonstrated the in vitro antioxidant potential of quinoa seeds extracts,providing scientific basis for the estimation of the in vivo antioxidant activities of quinoa seeds.