超声波-阴/阳离子表面活性剂协同提取茅岩莓总黄酮的工艺研究

朱欣; 郭红英; 谭兴和; 于潇杰; 张喻; 王锋; 邓洁红

doi:10.13386/j.issn1002-0306.2016.01.035

超声波-阴/阳离子表面活性剂协同提取茅岩莓总黄酮的工艺研究

Ultrasonic- assisted anionic and cationic surfactant systems extraction of total flavonoids from Ampelopsis grossedentata

摘要

摘要: 优化超声波协同阴/阳离子表面活性剂提取茅岩莓总黄酮的工艺,通过单因素实验,考察表面活性剂浓度、阴/阳离子表面活性剂摩尔质量比、提取温度、乙醇浓度和提取时间对茅岩莓总黄酮得率的影响。在此基础上,固定阴/阳离子表面活性剂摩尔质量比为40∶1(SDS∶CTAB)、料液比1∶20(g/m L)和提取温度60℃,采用响应面实验考察表面活性剂浓度、乙醇浓度和提取时间对茅岩莓总黄酮得率的影响。结果表明最佳提取工艺参数为:表面活性剂浓度1.1%、乙醇浓度40%、提取时间57 min,此条件下茅岩莓总黄酮得率为23.27%,与预测理论值23.45%相差较小,说明该实验方法可行。

Abstract: The ultrasonic- assisted anionic and cationic surfactant systems extraction of total flavonoids was optimized by single- factor experiment and response surface methodology.The effects of surfactant concentration,the ratio of anionic surfactant to cationic surfactant,ultrasonic treatment time,solid / liquid ratio( g / m L) and temperature on the yield of total flavonoids were explored through single factor.Based on the optimum conditions of single factor experiment as SDS ∶ CTAB = 40 ∶ 1、1 ∶ 20 and 60 ℃ for the ratio of anionic surfactant to cationic surfactant,solid / liquid ratio( g / m L) and temperature,The optimum extraction conditions were determined as 1.1%,40% and 57 min for surfactant concentration,ethanol concentration and extraction time,respectively.Under these conditions,the yield of total flavonoids from Ampelopsis grossedentata was 23.27%,which was close to the predicted value of 23.45%.Therefore,the optimized extraction procedure was reliable.