响应面分析法优化超临界CO2萃取蓝莓花色苷工艺条件的研究
Optimization of supercritical CO2 extraction of blueberry anthocyanins using response surface methodology
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摘要: 以蓝莓为原料,利用响应面法优化超临界CO2萃取蓝莓花色苷的工艺。在单因素实验基础上,以萃取压力、萃取温度、萃取时间和液料比为影响因素,应用Box-Behnken方法进行四因素三水平实验,以花色苷含量为响应值,进行响应面分析。确定萃取的最佳工艺条件是萃取时间60 min,萃取温度40℃,萃取压力28 MPa,液料比为7∶1（m L∶g）,花色苷含量的预测值为1.59 mg/g,验证值为1.58 mg/g。结果表明利用响应面分析方法对超临界CO2萃取蓝莓花色苷的工艺条件进行优化,可获得最优的工艺参数,从而为进一步的研究提供依据。Abstract: To optimize supercritical carbon dioxide extraction of anthocyanins from blueberry using response surface methodology based on a 4- variable,3- level Box- Benhnken design.The results showed that the optimum conditions were extraction pressure of 28 MPa,extraction temperature of 40 ℃,extraction time of 60 min and liquid materials ratio of 7 ∶ 1( m L ∶ g). Under these conditions,the model predicted and experimental values of anthocyanins content were 1.59 mg / g and 1.58 mg / g,respectively.
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