Abstract: Taken Parastichopus californicus as raw materials,the technologies for hydrolyzing sea cucumber were optimized.The approximate compositions of the raw materials were analyzed. The results showed that the protein content was 73.33%. The lipid content was 0.97%. The ash content was 23.85%. The mucopolysaccharide content was 1.68%.The enzymes used in the experiment were the bacillus subtilis neutral proteinase and the flavourzyme.The technologies for hydrolyzing the sea cucumber were as the hydrolyzing temperature 55 ℃,p H 7.5,the material to liquid ratio 1∶ 5( g / m L),the hydrolyzing time 3 h,the amount of enzymes 1.05%.The peptide content of enzymatic hydrolysis at the optimal conditions was 13.99%.The polypeptide molecular weight distribution of their hydrolyzing products were estimated by Sephadex G- 50,which indicated that the distribution of Parastichopus californicus was among 1133~129457 u.