Abstract: Objective: In order to make lentinus edodes protein easylier absorbed and improved the utilization of lentinus edodes protein in the body. Methods: With lentinus edodes as raw material,lentinus edodes prepared peptide by enzymatic method.The peptide yield was took as the index in the experiment,which had a comparison of the enzymatic hydrolysis' effect with pepsin,3.350 acid protease enzyme,pineapple proteinase,papain and a variety of other enzymes. Results: The best conditions of preparation for lentinus edodes peptide was enzyme quantity 3000 U / g,p H 9.0,temperature 45 ℃,enzymolysis time 4 h,and the enzymatic hydrolysis' yield was the92.7%.G25 gel chromatography analysis showed that the peptides' molecular weights were mainly two parts of1000 ~5000 u and < 1000 u,the oligopeptide's proportion of molecular weight < 1000 u accounted for 68.38% of total polypeptide.From the amino acid analysis of lentinus edodes peptide,it retained most of protein's nutrients.Conclusion: The effect of enzymatic hydrolysis with trypsin was the best. With the best condition,nutirents were adequately retained,most of lentinus edodes peptide was oligopeptide and can be absorbed well by human body.