水溶性黑木耳根多糖对四氯化碳所致小鼠肝损伤的保护作用

郭国丽; 陈悦; 佟长青; 曲敏; 李伟

doi:10.13386/j.issn1002-0306.2015.24.066

水溶性黑木耳根多糖对四氯化碳所致小鼠肝损伤的保护作用

A water-soluble polysaccharide from Auricularia auricula root and its hepatoprotective effect against acute carbon tetrachloride-induced liver injury in mice

摘要

摘要: 研究黑木耳根多糖的特性及其对四氯化碳（CCl₄）所致小鼠肝损伤的保护作用。采用热水浸提及乙醇沉淀方法从黑木耳根中提取水溶性粗多糖（AARP）。采用分子筛层析法测定所提取的AARP的相对分子量约为8.6×105 u。HPLC层析分析AARP由6种单糖组成,其甘露糖∶鼠李糖∶葡萄糖∶半乳糖∶木糖∶岩藻糖的摩尔比为3.389∶0.406∶7.003∶1.248∶1.405∶4.86∶0.45。与模型组相比,AARP中（50 mg/（kg·d））、高（100 mg/（kg·d））剂量组可极显著抑制CCl₄所致小鼠肝损伤的血清中AST活性的升高,极显著降低了肝组织中MDA的含量,并且抑制肝组织中SOD活性的下降。结果表明,AARP对CCl₄所致小鼠肝损伤具有保护作用。

Abstract: To study the characteristic of the Auricularia auricula root polysaccharide(AARP) and protective effect of AARP on acute carbon tetrachloride-induced liver injury. A water-soluble AARP was isolated by hot-water extraction and 80%(v/v) ethanol precipitation. Based on the calibration with Dextran,AARP had a molecular weight of approximately 8.6×105u. The quantification of monosaccharides of AARP was analyzed using highperformance liquid chromatography. According to the corresponding relationship of peak and migration time,AARP mainly consisted of six kinds of monosaccharides apparently. The mole ratio of mannose,rhamnose,glucose,galactose,xylose and fucose of AARP were calculated as 3.389 ∶0.406 ∶7.003 ∶1.248 ∶1.405 ∶4.86:0.45.The protective effect of AARP on acute carbon tetrachloride-induced liver injury was investigated in mice.Compared with modle control group,treatment with middle(50 mg/(kg·d)) and high(100 mg/(kg·d)) doses of AARP were significantly decreased serum aspartate aminotransferase(AST) and malondialdehyde aldehyde(MDA) levels,increased superoxide dismutase(SOD) activity,and improved hepatic injury in the acute model of liver injury in mice. These results suggest that AARP possesses potent hepatoprotective activity.