Abstract: Objective : The improvement effect of refined polysaccharide from Boletus aereus( RPBA) on acutealcoholic hepatic injury in mice was investigated. Methods:The 60 mice were fed adaptively for 3 days,then were randomly divided into six groups,including blank control group,alcoholic model group,positive control group(administered with 150 mg/kg·bw bifendate by gavage),and RPBA(100,200,400 mg/kg·bw)group. After housing for 30 days,the mice were kept fasting but retained water for 12 hours,then 50% alcohol(12 m L/kg·bw) were administered orally into animals of all groups except blank control group to induce hepatotoxicity. Results:The results showed that the levels of ALT,AST,TG and MDA of mice in model group were incereased obviously(p <0.05),while the levels of SOD,CAT,GSH-Px,and GSH were lower(p <0.05)compared with the control group. After BAP administration,the levels of AST,ALT and MDA were decreased while the levels of SOD,CAT,GSH-Px,and GSH were increased. Conclusion:RPBA could significantly reduce the levels of AST,ALT and TG in serum of mice,increase the activities of antioxidant enzymes in serum,and reduce lipid peroxidation.RPBA could protect liver from injury induced by alcohol in mice.