Abstract: In this study,17 stains were selected from the Chinese traditional fermented food by colonial morphology,gram staining and soluble calcium transparent circle. 16 S r DNA sequencing technology was used to confirm their taxonomy status. Their ability of acid resistant and bile salt resistant were tested. Four strains,WHLP-01,WHLP-02,WHLP-03 and WHPE-02,could survived under the environment of p H 2 for two hours. The bile resistance experiment results showed that the WHLP-01 and WHLP-02 strains could survived under ambient of 0.3% bile salt concentration. In addition,it was found that Lactobacillus plantarum WHLP-01 had a significant inhibitory effect on the eight common pathogens,and had a good ability to reduce cholesterol. Further studies should be performed on as micro ecological agents.