Abstract: In this experiment,traditional method of isolation and cultivation was adopted to get excellent Daqu yeast strains. 89 dominant yeast strains had been isolated from the spring medium-high-temperature Daqu of some liquor-making enterprise in the north of Anhui province,and then 6 better-growing strains were chosen for secondary screening. Finally 2 representative yeasts strains(N4,N6),which had preponderant growth and stronger alcohol-producing abilities,were picked out through several conventional methods of analysis such as bacterial colony,cellular morphology,features of physiology and biochemistry. After using 18S-ITS r DNA sequencing and phylogenetic analysis, researchers identified N4 as Cryptococcus magnus, and N6 as Sporidiobolus pararoseus. They belonged to the genus of Sporidiobolus. The 2 selected yeasts strains were important strains resources for liquor-production in the north of Anhui province and they were of importance in application.