Abstract: With two kinds of bacteria(Bacillus subtilis W1-3,Bacillus subtilis 10160) and three kinds of fungi(Pichia,Saccharomyces cerevisiae and Aspergillus niger) as starting strains,rapeseed meals as raw material,glucosinolate( thioglycoside) degradation rate and peptide recovery ratio as quality evaluation index of fermentation meal,single bacteria and mixed bacteria solid-state fermentation experiment was carried out.The result showed that the optimal single bacterium fermentation strain was Bacillus subtilis W1-3. Comparing the Bacillus subtilis W1-3 single bacteria fermentation with its mixed fermentation with other one or another two kinds of bacteria,its single bacterium fermentation was better than that of mixed bacteria fermentation. After fermentation by Bacillus subtilis W1-3,it was found that the content of anti-nutritional substances such as glucosinolates and phytic acid decreased 62.14% and 31.58%,and useful substances such as peptides,crude proteins,whole amino acid and the number of living bacterium increased 453.47%,10.39%,17.76%.