反油酸和反异油酸对人脐静脉内皮细胞炎症反应的影响

文晓东; 刘本欣; 邓泽元; 范亚苇; 李静

doi:10.13386/j.issn1002-0306.2015.23.065

反油酸和反异油酸对人脐静脉内皮细胞炎症反应的影响

Exploration of elaidic acid and trans- vaccenic acid on inflammation of human umbilical vein endothelial cells

摘要

摘要: 比较反油酸（9t18∶1）和反异油酸（11t18∶1）对内皮细胞炎症反应的影响。用不同浓度的反油酸（9t18∶1）和反异油酸（11t18∶1）作用人脐静脉内皮细胞（HUVEC）,观察其对细胞存活率、细胞形态学、氧化酶活（SOD、MDA）、LDH渗出率、一氧化氮量和NOS活力的影响。结果表明9t18∶1和11t18∶1均能降低细胞存活率,在100μmol/L时,9t18∶1对内皮细胞存活率的抑制作用显著强于11t18∶1;两种反式脂肪酸均导致内皮细胞形态发生变化,细胞由正常的梭形变为圆形,而且边缘呈粗糙状态;9t18∶1和11t18∶1均能够造成内皮细胞LDH渗出率和胞内MDA含量的显著升高,但11t18∶1组明显低于同浓度9t18∶1组;两种反式脂肪酸均能够降低HUVEC细胞NO分泌量和NOS、SOD活力,且11t18∶1的降低作用低于同浓度9t18∶1组。此外,9t18∶1和11t18∶1均可提高ICAM、VCAM、IL-6 mRNA表达量,且前者显著高于后者。总的来说,这两种反式脂肪酸对内皮细胞都有一定致炎症作用,但9t18∶1强于11t18∶1。

Abstract: The aim of the present study was to compare the effects of the elaidic acid( 9t18 ∶ 1) and the trans-Vaccenic acid( 11t18 ∶ 1) on endothelial cells injury. After adding different concentrations of 9t18 ∶ 1 11t18 ∶ 1,the cell viability,cell morphology,oxidase activity( SOD and MDA),LDH leakage rates,effects of NO content and NOs activity were obserred.The results showed that 9t18∶1 and 11t18∶1 reduced the cell survival rate,and the inhibitory effect of 9t18∶ 1 on proliferation of endothelial cells were stronger than 11t18 ∶ 1 in 100 μ mol / L.Both of trans fatty acids lead to changes in endothelial cell morphology,cells from normal spindle to round and edge became rough.9t18∶1 and 11t18 ∶ 1 increased LDH leakage rate and MDA in endothelial cell,but the 11t18 ∶ 1- treated group was reduced than 9t18∶1 in the same concentration.NO secretion,NOS and SOD activity was reduced by 9t18∶1 and 11t18∶1,but 11t18∶1 group was significantly higher in the same concentration of 9t18 ∶ 1. Both of 9t18 ∶ 1 and 11t18 ∶ 1 could increase the mRNA expression levels of ICAM,VCAM,and IL-6,the former was obviously higher than the latter.In summary,the HUVEC cells injury induced by 11t18∶1 was significantly weaker than that of 9t18∶1.