蔗梢多酚类化合物抗氧化与抗肿瘤活性研究

何雪梅; 孙健; 李丽; 盛金凤; 赵谋明

doi:10.13386/j.issn1002-0306.2015.23.063

蔗梢多酚类化合物抗氧化与抗肿瘤活性研究

Antioxidant and antitumor activities of polyphenol compounds from sugarcane top

摘要

摘要: 研究从蔗梢中分离到的8种多酚类化合物的抗氧化活性和抗肿瘤活性。采用DPPH自由基清除能力、氧自由基吸收能力（ORAC）和ABTS自由基清除能力综合评价多酚化合物的体外抗氧化活性,MTT法评价多酚化合物的体外抗肿瘤活性。实验表明,8种化合物中槲皮素的DPPH自由基清除能力显著（p<0.05）高于阳性对照Trolox;咖啡酸抑制CNE2（人鼻咽癌细胞）增殖的活性最强,槲皮素抑制SGC7901（人胃癌细胞株）增殖的活性最强,2-（3,5-二羟苯基）-5-羟基-苯并呋喃和槲皮素能较强地抑制Hela（人宫颈癌细胞株）的增殖。蔗梢中的8种多酚类化合物均具有较强的抗氧化活性和一定的抗肿瘤活性,可作为保健食品和保健品的开发原料。

Abstract: The antioxidant and antitumor biological activities of eight polyphenol compounds which were separated from sugarcane top were studied.The test results showed that DPPH radical scavenging activity of quercetin was significantly higher( p < 0.05) than that of positive control Trolox.Caffeic acid showed the strongest inhibiting activity on CNE2 proliferation. Quercetin exhibited the strongest inhibiting activity on SGC7901 proliferation. 2-( 3,5- Dihydroxy phenyl)- 5- hydroxy- benzofuran and quercetin possessed stronger inhibiting activity on Hela proliferation.Eight polyphenolic compounds of sugarcane top exhibited good antioxidant activities and antitumor activities,indicated that sugarcane top would be good material for health products and health foods.