Abstract: Objective: Using HPLC to study the serum metabolites of antidiabetic diabetes effect of Xinjiang fermented camel milk,and compared the change of the amino acid content of fresh and fermented camel milk.Methods: high sugar and high fat feed eight weeks and injected STZ with 30 mg·kg- 1to establish the model of diabetic rats,then the rats were randomly divided into five groups,they were given fermented camel milk low,medium and high dose and Sitagliptin,normal group rats given fresh camel milk. After four weeks of treatment,HPLC to determinate the content of serum free amino acid.Results: Compared with the normal group,model group rats serum aspartic acid,increase the content of phenylalanine,threonine and tyrosine content reduced.Fermented camel milk in middle dose group can reduce the content of aspartic acid,phenylalanine,glycine,arginine content increased.Fermented camel milk in high dose group significantly increased serum levels of arginine. Sitagliptin group can reduce the serum aspartic acid content increased,glycine,arginine,leucine and isoleucine content.Conclusion: fermented camel milk middle and high dose group can decrease the content of diabetes rats serum aspartate and phenylalanine,increase the content of glycine and arginine,adjust the serum amino acid metabolic disorder of diabetes rats.