Abstract: A method was established by Ultraviolet spectrophotometer for the determination of gingerol in ginger oil extracted by the super critical CO₂.The solubility and wavelength scanning experiments showed that the solubility of ginger oil was well in anhydrous ethanol,and the maximum absorbance value was at 278 nm. With anhydrous ethanol as solvent at 278 nm,the calibration curves had good linearity in the range from 2.011 to12.066 μg / m L( R2= 0.9999).The average recovery rate of gingerol was 98.76 % ~ 101.21 %( RSD = 1.05 %).The method of Ultraviolet spectrophotometer was quickly and stable as the apparatus required were simple and easy to operate.