微波提取白鹃梅黄酮类物质及其抗氧化活性研究

朱玲玲; 张广文; 杨婷婷; 邱瑞霞

doi:10.13386/j.issn1002-0306.2015.23.041

微波提取白鹃梅黄酮类物质及其抗氧化活性研究

Study on the microwave extraction technology of flavonoids from Exochorda racemosa and the antioxidant evaluation

摘要

摘要: 本文以白鹃梅为原料,采用微波辅助提取白鹃梅中黄酮类化合物。在单因素实验的基础上,利用响应面法对微波功率、时间、料液比、温度进行优化;并对其体外抗氧化活性进行测定。结果表明,微波功率、时间、温度对总黄酮提取量影响比较显著;最佳工艺条件为:微波功率357 W,时间63 min,料液比1∶26 g/m L,微波温度47℃,由此得到白鹃梅总黄酮提取量为14.59 mg/g,与预测值14.67 mg/g的相对误差为0.5%。总黄酮清除DPPH·、·OH、O-2·以及的IC50值分别为0.52、0.55、1.18 mg/m L。其清除DPPH·、·OH的能力高于BHT,而低于VC;清除O-2·的能力均低于VC、BHT。结论:微波辅助提取法能够提高白鹃梅中总黄酮提取量,且白鹃梅中黄酮类物质具有一定能力的抗氧化活性。

Abstract: Response surface methodology( RSM) was used to optimize the ultrasonic extraction conditions of lavonoids from Exochorda racemosa based on the Box- Behnken design. The factors of microwave power,time,solid to liquid ratio,microwave temperature were analyzed and vitro antioxidant activity were measured.The results showed that microwave power,time and temperature had significant effect on flavonoids yield. The optimum extraction conditions were as follows: microwave power 357 W,extraction time 63 min,ratio of solid to liquid 1∶26 g / m L,microwave temperature 47 ℃. Under the optimized conditions,the yield of flavonoids was 14.59 mg / g,compared with predictive yield which was 14.67 mg / g,the relative error was 0.5%.The value of IC50 of DPPH·,·OH,and O₂^-·were 0.52,0.55,1.18 mg / m L. The scavenging effect of DPPH·,·OH was higher than BHT,but lower than VC.The scavenging effcct of O₂^-· were all lower than VCand BHT.Conclusion: the yield of flavonoids was higher by the methods of microwave extraction and the flavonoids in Exochorda racemosa had got certain ability to antioxidant evaluation.