Abstract: In order to increase the bacterial cellulose( BC) productivity of Acetobacter xylinum using pomace hydrolysate,response surface methodology was used to optimize the fermentation medium. Meanwhile,the properties of BC were compared through fourier transform infrared( FT- IR) and X- ray diffraction( XRD).According to the results of single factor experiments and response surface experiments,the optimal fermentation medium were determined as follows: saccharose 38.44 g,peptone 10.91 g,magnesium sulfate 0.85 g,xanthine 0.87 g,ethanol 10 m L,pomace hydrolysate 1000 m L and p H6.0,under these conditions,the BC productivity was 7.19 g / L,increased by 27.3% in comparision to before( 5.65 g / L). The properties and structures of BC produced with pomace hydrolysate were confirmed to be basically same with BC produced with basic medium,which indicated that the pomace hydrolysate could be used as part of raw materials to ferment BC and would not affect the properties of BC.