海洋低温α淀粉酶水解玉米淀粉及其产物生物活性

Hydrolyzate of corn starch by marine cold- adaptedα-Amylase and its biological activity

摘要: 本研究旨在开发适用于工业生产的新型酶制剂,采用研制的新型海洋低温α-淀粉酶,研究该酶水解玉米淀粉最佳工艺条件以及其产物特性。低温α-淀粉酶最佳的工艺条件为温度30℃、时间90 min、淀粉浓度4.5%、加酶量8 U/g、p H6.5。酶解产物通过TLC和HPLC分析,酶解产物中主要为麦芽糖、麦芽三糖、异麦芽三糖、麦芽四糖和麦芽五糖,其中麦芽四糖和麦芽五糖的和达到69.62%。酶解产物对羟自由基和DPPH自由基均有清除作用,对羟自由基的清除效果要好于对DPPH自由基的清除。

Abstract: The purpose of this study was to develop a new enzyme for industrial production.A kind of marine coldadapted α- amylase was adopted to hydrolyze corn starch. Some factors which could influence enzymatic hydrolysis were studied.The optimal conditions of corn starch hydrolysis with the α- amylase: temperature 30 ℃,hydrolysis time 90 min,substrate concentration 4.5%,enzyme amount 8 U / g and p H6.5. The amylase could hydrolyze raw starch into maltose,maltotriose,isomaltotriose,maltotetraose and maltopentaose by TLC and HPLC of analysis. The quantity of maltotetraose and maltopentaose was 69.62%. The hydrolyzate had the scavenging effect on the hydroxyl radical and DPPH radical.The scavenging effect of the hydroxyl radical was better than the DPPH radical.