Abstract: The clearance effect of total flavonoids from Illicium verum( FIVR) on ·OH,O₂^-· were determined using Fenton method and compared with that of vitamine C.Total antioxidant capacity( T- AOC) were determined using FRAP method to evaluate FIVR in vitro antioxidant activity. The aging mice model was established by the intraperitoneal injection of D- galactose.The mice were randomly divided into normal control group,model group,positive control group,FIVR high,medium and low- dose groups( 200,100,50 mg / g·d). After administrations for48 d,the body weight of mice were determined,then the organ coefficients were counted. The content of SOD,GSH- Px of liver,brain tissue and in the blood was detected. From FIVR antioxidant experiment in vitro,the clearance rate of ·OH,O₂^-· and T- AOC were increased with the increase of concentration.The body weight of mice,liver index,brain index and the activity of SOD,GSH- Px in liver tissue,brain tissue of model group were lower than normal group,which proved successful model; each FIVR dose group mice weight,liver index,brain index and the activity of SOD,GSH- Px in liver tissue and brain tissue were higher than model group. This study shows that FIVR has obvious antioxidation activity.