Abstract: From the perspective of metabolism,fresh-keeping actually was to reduce catabolism of fruits and vegetables in their living condition so that their shelf life were prolonged,in-depth research of the law of metabolic flux will achieve the purpose better. This study used melon as a test subject discussed the law of metabolic flux of fruits and vegetables during their storage. In order to obtain the metabolic flux of the following pathways:embden meyerhof pathway(EMP),pentose phosphate pathway(PPP),sucrose synthesis pathway(SSP) and TCA cycle(TCA),melon was stored at different temperatures(0,5,8,10,20,30,40,45,50,55 ℃).The results showed that sucrose had a higher synthesis flux at the temperatures of 8 ℃ and 50 ℃,and lactate had a higher metabolic flux at the temperature of 8 ℃. Thus,8 ℃ was the optimal storage temperature. These findings would be enable to provide the reference for the in-depth study fruits and vegetables storage from the perspective of metabolic,improve the quality of the fruit store.