Abstract: The vibration spectrum characteristics of normal pear and rough peel pear were tested by pulse excitation,which were to realize the identification of rough peel pear among the normal pears. The difference significance of response dominant frequency between the normal and the rough peel ones was analyzed. The statistics of content of stone cells were analyzed. The microstructures of peel and flesh tissue of two kinds of pear were observed. The results showed that the dynamic signals were stable and reliable at the same detection point. Also,the responses of dynamic spectrum were consistent at different excitation points on the cheek and the line of equator with single excitation and detection. Response dominant frequency of the rough peel and the normal pear were(687.5±13.1) Hz and(775±19.4) Hz,respectively and there was significant(p <0.05) difference of dominant response frequency. Main reasons of the rough peel pear with the bigger response dominant frequency were that there were compact epidermis,lots of bonny cell,lower water content(wet base),a number of stone cell(mass) and lignocellulose widely distributed in flesh of the rough peel pear.