气相色谱法测定自酿葡萄酒中甲醇的含量

郑亚琴; 邱艳丽; 汪偶; 臧鑫; 李倩

doi:10.13386/j.issn1002-0306.2015.22.002

气相色谱法测定自酿葡萄酒中甲醇的含量

Determination of methanol in self-made wine by gas chromatography

摘要

摘要: 目的:针对人们所担忧的葡萄酒中甲醇含量超标的问题,本实验建立一种测定自酿葡萄酒中的甲醇含量的方法。方法:采用气相色谱法对葡萄酒中的杂质甲醇进行定量检测。色谱条件:固定相为Gs BP-Ino WAX毛细管柱,柱温为90℃,载气为氮气,流速为1.4 m L/min;以氢焰离子化检测器（FID）为检测器,温度为200℃;进样口温度为150℃。结果:该方法在16¹000μg/m L的浓度范围内线性关系良好（R2=0.9987）,精密度为1.2%,回收率为90.03%⁹4.00%。结论:该方法快速准确,分析成本低,能够满足快速分析自酿葡萄酒中甲醇的要求。

Abstract: Objective:The aim of this investigation was to develop a method to detect methanol quantitatively in self-made wine. Methods :Using gas chromatography for quantitative detection of methanol in wine. The chromatographic conditions :stationary phase was capillary column of Gs BP-Ino WAX,column temperature :90 ℃,carrier gas:nitrogen,flow rate:1.4 m L/min;hydrogen flame ionization detector(FID) as the detector,the temperature was 200 ℃;the inlet temperature was 150 ℃. Results:The data showed that in the concentration range of 16 ~ 1000 μg / m L, a good linear relationship( R2= 0. 9987). The method was validated with a good accuracy and the relative standard deviation( RSD) was 1. 2 %. Recovery was between 90. 03 % ~ 94. 00 %.Conclusion : Quick and relatively low- cost method was developed, and the method was appropriate for quantitative analysis of methanol in self-made wine.