黑木耳甲醇提取物抑制黑色素的研究

Study on extraction of methanol and inhibition of melanin from Auricularia auricular

摘要: 为确证黑木耳与黑色素的关系,鉴于已有的研究,以甲醇为溶剂对黑木耳进行提取,提取物采用TLC检测与化学显色鉴别相结合的方法,明确其组成的物质类别;并以熊果苷为阳性对照,以鼠黑色素瘤B16细胞株作靶标,采用A405测定黑色素含量,MTT法测定细胞增殖能力,对提取物进行黑色素抑制活性研究和细胞毒性评价。结果显示,黑木耳甲醇提取物含有多酚、萜类、多糖和氨基酸;此提取物在浓度较高时,具有黑色素抑制活性和较低的细胞毒性。

Abstract: To explore the relationship between melanin and Auricularia auricular, according to existing research, taking methanol as solvent, the sort of compound in extraction was identified by TLC etc.B16 melanoma cells were used as indicator cells, melanin content was determined by measuring the absorbance at 405 nm.Cell viability or cytotoxicity effect was assayed by determining the number of living cell using MTT assay and comparing with arbutin.The results showed that Auricularia auricular extract had polyphenols, terpenoids, polysaccharides and amino acids, and it had strong inhibitory effect on melanin biosynthesis and had low cytotoxicity to B16 melanoma cells when the concentration was high.