牛磺酸对丙烯酰胺致SH-SY5Y细胞损伤的保护作用

郝瑞芳; 景浩

doi:10.13386/j.issn1002-0306.2015.20.066

牛磺酸对丙烯酰胺致SH-SY5Y细胞损伤的保护作用

郝瑞芳,
景浩

Protective effects of taurine on SH-SY5Y cells injury induced by acrylamide

摘要

摘要: 用丙烯酰胺诱导SH-SY5Y神经细胞损伤建立细胞损伤模型,研究牛磺酸对SH-SY5Y细胞的保护作用。MTT法和LDH法测定结果显示:用100⁷00μg/m L丙烯酰胺对SH-SY5Y细胞孵育8 h,细胞存活率下降至52%⁸1%,表明丙烯酰胺对SH-SY5Y细胞有一定的损伤作用,导致细胞膜受损,LDH释放到细胞膜外。经牛磺酸（50、100、200μg/m L）预先孵育1 h后,再用300μg/m L丙烯酰胺对细胞孵育8 h,SH-SY5Y细胞的存活率分别升高至81.6%、92.7%、89.7%,显著高于丙烯酰胺损伤组的细胞存活率（67.6%）;且LDH释放程度呈现出下降趋势,LDH释放率分别为:150.7%、138.8%、113.3%,和丙烯酰胺损伤组的LDH释放率（188.5%）相比均显著降低。该研究结果表明牛磺酸对丙烯酰胺诱导的SHSY5Y细胞损伤具有保护作用。

Abstract: The preventive effects of taurine on acrylamide-induced cell injuries of human neuroblastoma cells(SH-SY5Y) were investigated by using cellular morphology,MTT assay and LDH assay. The results showed that the cell viabilities were decreased to 52%⁸1% when SH-SY5 Y cells were incuvated 8 h with acrylamide of 100 ⁷00 μg/m L,which showed that acrylamide induced the cell membrane damage and LDH release outside cell membrane. The cell viability was decreased to 67.6% and LDH release rate was increased to188.5% when SH-SY5 Y cells were only incubated 8 h with acrylamide of 300 μg/m L in acrylamide injury group.However,when SH-SY5 Y cells were pre-protected 1 h with taurine of 50,100 and 200 μg/m L before incubation8 h with acrylamide of 300 μg/m L,taurine resulted in significant increases of SH-SY5 Y cell viability to 81.6%,92.7% and 89.7%,and decreases of LDH release to 150.7%,138.8% and 113.3% as compared with that in acrylamide injury group. These demonstrated that taurine could protect SH-SY5 Y cells from acrylamide-induced injury.