一株绿色素产生菌的筛选及鉴定

左勇; 傅彬; 叶碧霞; 江鹏; 王小龙; 杨小龙

doi:10.13386/j.issn1002-0306.2015.20.031

一株绿色素产生菌的筛选及鉴定

Isolation and identification of one strain of stretomyces producing green pigment

摘要

摘要: 目的:从土壤中筛选绿色素产生菌株并对该菌株进行鉴定。方法:采用平板法分离产绿色素菌株,利用形态、生理生化及分子生物学的方法对菌株进行菌种鉴定。结果:从四川理工学院校园土壤中分离得到一株产绿色素的放线菌t9,在高氏一号培养基上,气生菌丝为灰白色,基内菌丝为绿色。该菌株基因序列与鼠灰链霉菌Streptomyces_murinus_NBRC₁2799（T）相似度为99%,以Neighbor-Joining（N-J）法构建的系统发育树。结论:菌株t9的16S r DNA序列分析结果结合形态学特征及生理生化鉴定,确定该菌株为鼠灰链霉菌属（Streptomyces_murinu）,并将其命名为Streptomyces_murinus t9。

Abstract: Objective:In order to isolation and identification of strain of producing green pigment. Methods:The microbial strains of producing pigment were isolated from soil by method with plate coating,biochemical and morphological characteristics and 16 S r DNA gene sequence analysation. Results:An actinomycete,named t9,was isolated from a soil sample in the College of Bioengineering,Sichuan University of Science and Engineering.It produced grayish-white aerial mycelium and green substrate mycelium on Gause 's synthetic agar. The results showed that the 16 S r DNA gene sequence of the train t9 shared the identity 99% with that of Streptomyces_murinus_NBRC₁2799(T),the phylogenetic tree was derived with NJ. Conclusion:The strain t9 was identified as Streptomyces_murinus from its morphological andcultural characteristics and the 16 S r DNA gene sequence.