15种红果肉海棠多酚及抗氧化活性分析

魏新翠; 李厚华; 蔡雪雁; 梁峥; 唐豆豆

doi:10.13386/j.issn1002-0306.2015.20.019

15种红果肉海棠多酚及抗氧化活性分析

Polyphenols and antioxidant activity analysis of 15 red flesh crabapples

摘要

摘要: 以15种红果肉海棠为材料,采用清除二苯代苦味酰基（DPPH）自由基、清除2,2′-连氨-（3-乙基并噻唑啉-6-磺酸）二氨盐（ABTS）自由基法对其进行了抗氧化活性评价。Folin-Ciocalteau法测其多酚含量,高效液相色谱（high performance liquid chromatography,HPLC）分析其酚类物质的组成与含量,相关系数法分析多酚、黄酮与抗氧化活性间的相关性。结果表明,其多酚含量为318.14⁸38.83 mg GAE/100 g干重,黄酮含量为367.64¹821.37 mg RE/100 g干重。印第安魔力海棠、红巴伦海棠、红园海棠和印第安夏天海棠的多酚和抗氧化能力均较高。检测出矢车菊素-半乳糖苷、矢车菊素-阿拉伯糖苷和芦丁等10种多酚类物质。

Abstract: 15 kinds of red flesh crabapples were taken as materials. 1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging, 2,2 '-azino-bis(3-ethylbenzothiazoline)-6-sulphonic aciddiamonium salt(ABTS+·) radical scavenging were carried out in antioxidant activity evaluation. The content of polyphenols were measured with the Folin-Ciocalteau method. HPLC-DAD was applied to test the composition of polyphenols. The results showed that their polyphenols contents were 318.14 ⁸38.83 mg GAE per 100 g of dry fruit and the flavones were 367.64¹821.37 mg RE per 100 g of dry fruit. M. ‘Indian Magic',M. ‘Red Barron',M. ‘Red Garden' and M. ‘Indian Summer' were the varieties that contained higher polyphenol,flavones and antioxidant capacity.A total of 10 polyphenols,including cyanidin galactoside,cyanidin arabinoside and rutin were detected by HPLC.