嗜麦芽窄食单胞菌42-2降解黄曲霉毒素B1的效果研究

郝修震; 岳晓禹; 李长滨; 辛婷; 李军霞

doi:10.13386/j.issn1002-0306.2015.20.010

嗜麦芽窄食单胞菌42-2降解黄曲霉毒素B1的效果研究

Study on the degradation of aflatoxin B1 by Stenotrophomonas sp.42-2

摘要

摘要: 黄曲霉毒素B1（AFB1）对人类和家畜的健康危害很大。本文以嗜麦芽窄食单胞菌（Stenotrophomonas sp.42-2）为研究菌株,对其进行了急性毒理实验,并利用该菌的上清液、发酵液和胞外蛋白粗提取液分别进行了发霉玉米、饲料、生大黄、柏子仁和怀山药的黄曲霉毒素B1降解实验。结果表明:活菌制剂在2.56×1010 CFU个/m L剂量以下不会引起急性毒性反应。在菌株42-2的上清液、发酵液和胞外蛋白粗提取液的毒素降解实验中,三者对发霉玉米中AFB1的降解率分别为:74.90%、82.60%和65.40%;对饲料中AFB1的降解率分别为:77.60%、82.50%和71.20%;对生大黄中AFB1的降解率分别为:73%、78.10%和68.40%;对柏子仁中AFB1的降解率为:76%、79.50%和70.50%;对怀山药中AFB1的降解率分别为:65.30%、69.10%和61.10%。

Abstract: Aflatoxin B1(AFB1) does great harm to human and animal health. The test of the acute toxicity of the strain had been done. The degradation tests of the AFB1 in mould maize,feed,rhubarb,semen boitae and yam had been implemented by using the strains' culture supernatant,the fermentation broth and the extracellular enzyme extract respectively. The results showed that the microbe cells didn't cause the reaction of the acute toxic if the biomass was lower than 2.56×1010CFU/m L. The results of the degradation test of the AFB1 were as follows. In maize,the degradation rates were 74.90%,82.60% and 65.40% respectively. In feed the degradation rates were 77.60%,82.50% and 71.20% respectively. In rhubarb the degradation rates were 73%,78.10% and68.40% respectively. In semen boitae the degradation rates were 76%,79.50% and 70.50% respectively. In yam the degradation rates were 65.30%,69.10% and 61.10% respectively.