基于改良ERIC-PCR技术快速鉴别人参和西洋参

Study on rapid identification of Panax quinquefolius and Panax ginseng based on improved ERIC-PCR

摘要: 为建立快速、准确的鉴定人参和西洋参并测定二者在混合物中含量的方法,将ERIC-PCR（Enterobacterial Repetitive Intergenic Consensus-Polymerase chain reaction）引物改良进行温度梯度PCR扩增,分析人参（Panax ginseng C.A.Mey,PG）和西洋参（Panax quinquefolium L.,PQ）电泳条带并寻找其特异性条带,结果发现一对特异性引物ER1和EL2,当退火温度为46.0℃时,只出现2.8 kb条带为西洋参,而出现2.8 kb和3.4 kb条带为人参。通过对混合样品中3.4 kb条带的定量分析,可以准确的定量西洋参的含量,系统误差仅为5.2%,RSD为3.5%,说明该方法可以快速、准确的鉴定西洋参和人参,并且可以准确测定混合物中西洋参的含量。

Abstract: Studied on the rapid identification and quantification of PQ and PG using improved ERIC-PCR,and a pair of new primers(ER1 and EL2) was found. When the annealing temperature was 46.0 ℃,the genome DNA PCR results showed one specific 2.8 kb band for PQ while two bands of 2.8 kb and 3.4 kb repectively for PG.It also was possible to quantify PG in mixed PQ and PG sample by quantification of the 3.4 Kb band,and the systematic error was only 5.2% and RSD was 3.5%. The study showed that this method was rapid and accurate to identify PQ and PG and to quantify PG in mixed PQ and PG.