响应面方法优化聚球藻7002嗜铁素的发酵条件
Optimization of the fermentation conditions for siderophores from Synechococcus sp.PCC 7002 by response surface methodology
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摘要: 为提高聚球藻7002嗜铁素的产量,采用响应面方法对其发酵条件进行优化。在单因素实验结果的基础上,根据BOX-Behnken中心组合原则,设计温度、p H、碳源、氮源4因素3水平响应面实验,以嗜铁素的相对含量为响应值优化聚球藻7002的发酵条件。实验所得最优发酵条件为温度31.7℃,p H 8.28,葡萄糖添加量14.47 g/L,氯化铵添加量0.19 g/L。聚球藻7002在优化所得条件下发酵15 d后,嗜铁素的相对含量达到95.42%,优化前仅为64.53%,嗜铁素的相对含量提高47.87%。响应面优化嗜铁素发酵条件的效果是显著的,嗜铁素产量得到明显提高。Abstract: In order to improve the yield of siderophores from Synechococcus sp. PCC 7002,response surface methodology( RSM) was used to optimize the fermentation conditions. On the basis of single factor experiment,Box- Behnken RSM was employed to investigate the four factors( temperature,p H,carbon sources,nitrogen sources) on the yield of siderophores.The optimum culture conditions were as follows: the temperature was 31.7 ℃,p H was 8.28,the amount of glucose was 14.47 g / L,the amount of NH4 Cl was 0.19 g / L.Under these conditions the relative amount of siderophores from Synechococcus sp.PCC 7002 was 95.42% after 15 days fermentation while the other group without optimizing was just 64.53%.The relative amount of siderophores was increased by 47.87% after optimizing. The culture conditions obtained by RSM were effective and reliable,the yield of siderophores improved significantly.
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