Abstract: Abject :In order to explore a convenient and efficient preservation method,the effects of different modified atmosphere packings on fresh-maintaining of herba houttyniae was investigated. Methods:Under the modified atmosphere packing with initial compositions containing 8% O2 and 30% CO2,herba houttyniaes were storaged at different temperatures for 30 days so that the optimal storage temperature was selected. In addition,effect of different modified atmosphere packing on all kinds of biochemical indexes were further investigated during the storage of 30 days so that the best preservation condition could be known. Results:Compared between the different modified atmosphere packings,the modified atmosphere packing with relatively low carbon dioxide concentration in initial compositions significantly(p<0.01) sustained the activity of polyphenoloxidase( PPO) and decreased the electrical conductivity in solution. The modified atmosphere packing with initial compositions containing 8% O2 and 30% CO2significantly(p<0.01) decreased the contents of malondialdehyde increasing,inhibited the activity of catalase(CAT) and peroxidase(POD),sustained the stable contents of total phenolic and flavones. Conclusion:The best fresh-maintaining condition and optimal storage temperature of herba houttyniae was 8% O2+30% CO2 and 2 ℃ respectively.