Abstract: In order to optimize the method of tea seed oil enzymatic hydrolysis by ultrasonic-assisted. Based on the single factor experiments,LVK lipase dosage,initial p H value and reaction temperature & time were selected as trial factors,the hydrolysis rate of tea seed oil was the response value,a central composite design was used to obtain a mathematical regression model for optimize the parameters of tea seed oil hydrolysis by lipase under ultrasonic. The results showed that the optimal parameters were obtained as follows:ultrasonic power 220 W,stirring speed 800 r/min,oil to water ratio 1∶1.75(w/v),Ca Cl2 dosage 0.25%,LVK lipase dosage5.5%,temperature 49.5 ℃,initial p H value 9.4,reaction time 4 h. Under these conditions,the experimental hydrolysis rate was 83.15% and the model value predicted was 82.06% and a high efficiency of hydrolysis was exhibited in this study.