Abstract: In order to investigate the influences of temperature and p H value on the growth and mycotoxins production of various Fusarium species,a total of 12 strains,which isolated from wheat,maize,rice and barley,were studied in present study. The results showed that a wide range of 10~35 ℃ and p H3~11 suitable for their growth were observed,and the optimum temperatures and p H values range were 20 ~30 ℃ and p H6 ~8.Different types of mycotoxins including type-A trichothecenes,type-B trichothecenes,fumonisins and fusaric acid were produced by the Fusarium investigated in current study. The temperature for the mycotoxins production was in the range of 5~40 ℃. The mycotoxin production were significantly affected by the variation in p H values,and peracid(p H3~5) or parlkaline(p H9~11) conditions would inhibit mycotoxins synthesis. On the whole,the mycotoxins production profiles were various between different Fusarium species,and more effects of temperature and p H values were observed on the mycotoxins production than that on the growth of fungi. For most Fusarium strains,the optimum conditions for mycotoxins production were inconsistent with the Fusarium growth itself.