Abstract: Antibacterial activities of eleven kinds of oxygen-containing sulfide flavors were studied,and the effect of structure on their antibacterial activities was further investigated. The antibacterial activities of oxygen-containing sulfide flavors against Vibrio parahaemolyticus,Staphylococcus aureus,Listeria monocytogenes and Escherichia coli were determined by measuring the diameter of inhibition zone. The effect of structure on their antibacterial activities was further investigated according to a statistical significance test. At concentration of5 mol/L,3-(methylthio) propionaldehyde(MTPD),2-methyl-3-(methylthio) furan(MMTF),furfuryl isopropyl sulfide( FIPS) and methyl furfuryl disulfide( MFDS) showed antibacterial activities against all four kinds of pathogenic bacteria. The inhibitory effects of these sulfide flavors followed the order MTPD >MFDS>or≈FIPS>or≈MMTF. By comparing the antibacterial activities of eleven kinds of oxygen-containing sulfide flavors,bis( 2-methyl-3-furyl) disulfide( BMFDS) showed the highest inhibitory effect against Staphylococcus aureus followed by MTPD,whereas BMFDS showed no effect on the other three pathogenic bacteria. MTPD showed the strongest inhibitor effects on Vibrio parahaemolyticus,Listeria monocytogenes and Escherichia coli,and the best action was found for Vibrio parahaemolyticus. The minimal inhibitory concentration of MTPD against Vibrio parahaemolyticus was 2.44 mmol/L. These results suggested that the aldehyde group play an important role in the antibacterial activity of oxygen-containing sulfide flavors against Vibrio parahaemolyticus,Staphylococcus aureus,Listeria monocytogenes and Escherichia coli.