磁性分子印迹固相萃取食品中的四环素类抗生素残留

王雅群; 潘道东

doi:10.13386/j.issn1002-0306.2015.18.002

磁性分子印迹固相萃取食品中的四环素类抗生素残留

Magnetic molecularly imprinted nanocompsites combined with solid phase extraction detect tetracycline antibiotic residues

摘要

摘要: 采用表面分子印迹技术合成对四环素类抗生素具有特异性吸附性能的Fe3O4@Si O2@MIP核壳型纳米复合材料,经磁分离固相萃取-高效液相色谱（MSPE-HPLC）技术,同时测定样品中3种四环素类抗生素的残留。用扫描电镜（SEM）、透射电镜（TEM）和红外色谱（FT-IR）对其表征。该方法对于四环素、土霉素、金霉素的检出限分别为9.61、7.84、11.93μg/kg,且在线性范围内线性关系良好。最大吸附量为56.28 mg/g。该方法的平均加标回收率在93.71%¹00.88%之间,变异系数1.54%⁷.44%。与非分子印迹聚合物相比,Fe3O4@Si O2@MIP分子印迹聚合物的吸附特异性较强。

Abstract: In this study, Fe3O4@Si O2@MIP core- shell nanocomposite was synthesized by surface molecular imprinting technique. It had an ability of selective extraction and detection of tetracycline antibiotics. The magnetic solid-phase extraction combined with high performance liquid chromatography(MSPE-HPLC) detects three kinds of antibiotic residues simultaneously. The magnetic Fe3O4@Si O2@MIP core-shell nanocomposites was characterized by scanning electron microscopy( SEM), transmission electron microscopy( TEM) and Fourier transform infrared( FT- IR) analysis. The result showed that the limit of detection of tetracycline,oxytetracycline and chlortetracycline was 9.61,7.84 and 11.93 μg/kg,respectively;the maximum adsorption capacity of magnetic Fe3O4@Si O2@MIP molecularly imprinted polymers was obtained at 56.28 mg/g,its recoveries ranging from 93.71% to 100.88% were obtained with relative standard deviations in the range of 1.54%~7.44%.Compared with Fe3O4@Si O2@NIP,the selective adsorption of Fe3O4@Si O2@MIP was more capable.