Abstract: In this paper,Wheat germ,a by- product of flour milling,possesses high bio- active and multiple functional compositions,such as the translational apparatus,translation factors and enzymes,which are essential for protein synthesis.With the development of biotechnology in the post genome era,the advantages of wheat germ cell- free protein synthesis system were showed in the expression of structural,toxic and functional proteins. This system was widely applied in proteomics research and the biopharmaceutical field,which provide strong evidence for the importance of developing derived products of wheat germ.