Abstract: In order to investigate the influence of load training and fructo- oligosaccharides binding load training on cell immune function of the trained rats,the incremental load training rats models were built by using treadmill training.After the rats were treated with 1 g / kg of fructo- oligosaccharide solution gavage,the changes of the weights and the weights of spleens of bodies,the weights of spleens,the activities of the spleens lymphocyte,and the subsets of lymphocyte were determined.The results showed that compared with the control group and the load training rats,supplementary fructo- oligosaccharide could effectively increase the weights and the weights of spleens of the load training rats( p < 0.05),improve the activities of the spleens lymphocyte,and increase the levels of CD4+cell subsets and CD4+/ CD8+ratio,the differences were statistically significant( p < 0.05). Supplement FOS could effectively improve the cellular immune function of rats with increasing load training.