气相色谱-质谱法测定葡萄酒中33种农药残留

吴帅; 李湘南; 王开宇; 霍胜楠; 韩圣谦

doi:10.13386/j.issn1002-0306.2015.17.054

气相色谱-质谱法测定葡萄酒中33种农药残留

Determination of 33 kinds of pesticide residues in wine by GC- MS

摘要

摘要: 本研究采用气相色谱-质谱法对葡萄酒中33种农药残留进行检测分析,考察了提取溶剂、提取方式及色谱条件对样品检测的影响。结果表明,采用丙酮/二氯甲烷超声提取结合气质联用检测技术对葡萄酒中33种农药残留进行检测,农药组分的回收率在65.2%¹21.2%之间,相对标准偏差在5.9%¹4.9%之间。回收率情况表明,绝大多数农药组分测试结果较好,个别项目处于可以接受的范围之内,该方法可以用于葡萄酒中33种农药的定性和定量分析。采用本方法对市场购买的47个葡萄酒样品进行检测,样品农残检出率为6.4%,检出项目有甲霜灵、嘧霉胺、氟硅唑和咪鲜胺。

Abstract: In this study,33 kinds of pesticide residues in wine were determined using gas chromatography- mass spectrometry( GC- MS). The factors of extraction solvent,extraction method and chromatography condition were investigated. The results showed that,using acetone / dichloromethane extraction with GC- MS technique,the recoveries of pesticides were from 65.2% to 121.2%,and the relative standard deviations( RSD) were within 5.9% ~14.9%. That suggested the method could be used for the qualitative and quantitative analysis of 33 kinds of pesticides in wine.Pesticide residues of 47 Wine samples bought in the market were determined according to this method,and the sample pesticide residues detection rate was 6.4%,the detection of pesticide residues including metalaxyl,pyrimethanil,flusilazole and prochloraz.