Abstract: The DHA algae oil and compound wall materials were used to prepare DHA algae oil the micro capsule powedr through emulsification,homogeneous,spray drying process.Taking 50% DHA algae oil as core material,whey protein isolate as the main wall material,the surface oil as index,the single factor experiment and the BoxBehnken design method were used for the DHA algae oil the micro capsule powder formula optimization.According to the influencing factors and the regression equation between response value of the best DHA algae oil the micro capsule powder formula: 31.5% whey protein isolate,arabic gum 11%,lactose 5%,core material DHA algae oil35%,1.76% casein,monoglycerides 2.14%,maltodextrin 13.6%. Prediction of DHA algae oil microcapsule powder surface oil was 0.34%,after three parallel experiments,test value of 0.28 lower than the industry standard 1.0%,the embedding rate could reach 98.78%,the fuel load was 35%. So this research was feasible,and had a certain reference to the production practice.