中性蛋白酶酶解缢蛏制备多肽工艺条件优化

Optimization of the hydrolytic conditions for the preparation of peptides from Sinonovacula constricta by neutral protease

摘要: 本实验利用中性蛋白酶对缢蛏进行酶解制备多肽,以多肽含量为指标,在单因素实验基础上采用正交实验对鲜缢蛏的酶解工艺进行优化。结果表明,中性蛋白酶最优酶解工艺条件为:p H7、酶添加量1200 U/g、料液比(m∶v)为1∶2.5、温度40℃、时间2 h,在此工艺条件下多肽含量为(79.2±0.7)mg/g。

Abstract: This research aimed at optimizing the hydrolyzation conditions of Sinonovacula constricta by neutral protease to prepare polypeptides.On the basis of single factor experiment,the orthogonal experiment was established and the yield of peptides was investigated to evaluate the effect of enzyme hydrolation.The optimal hydrolytic conditions could be concluded as follows: p H7,the dosage of neutral protease was 1200 U / g,the solid-liquid ratio was 1 ∶ 2.5( m ∶ V),the hydrolysis temperature was 40 ℃ and the hydrolysis time was 2 h.Under the optimal hydrolytic conditions,the yield of polypeptides was up to( 79.2 ± 0.7) mg / g.