冰葡萄酒酿造过程中糖分与乙醇变化的研究
Research of the changes of sugar and ethanol contents during ice wine brewing process
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摘要: 冰葡萄酒酿造过程中主要化学组分发生显著变化,尤其在主发酵期间糖分消耗和乙醇的产生,这些变化对冰葡萄酒的品质具有重要影响。本研究以威代尔(Vidal)冰葡萄汁为实验材料,接种酵母菌(ST)进行发酵酿制,利用HPLC-ELSD法测定冰葡萄酒发酵及陈酿过程中糖分含量的变化,同时检测乙醇浓度变化,酿造完成后进行微生物检测及感官风味评定。研究结果表明,冰葡萄汁中主要糖类为葡萄糖和果糖,在发酵过程中,葡萄糖含量从180.56 g/L下降至53.24 g/L,其中在第7 d到第28 d下降最快;果糖含量从223.65 g/L下降至160.54 g/L;乙醇浓度变化与葡萄糖含量变化呈负相关。最终成品的酒精度为11.0%v/v。冰葡萄酒在橡木桶中进行陈酿过程中,葡萄糖含量下降至47.64 g/L,果糖含量下降至151.25 g/L,酒精度下降至9.8%,微生物检测及感官评定均符合标准。本研究结果可为冰葡萄酒酿造提供理论依据,同时也为快速鉴定冰葡萄酒的真伪奠定了基础。Abstract: The main chemical constituents of ice wine changed significantly in the process of brewing,especially during main fermentation.These changes,including sugar's consumption and ethanol's production,had important influence on the quality of ice wine.This study took Vidal ice grape juice provided by Wunvshan Milan Wine Co.Ltd as material and inoculates Yeast( ST) to ferment.After fermentation,the ice grape juice was storaged in oak barrel for ageing which lasted 4 months.The changes of sugar and ethanol contents during fermentation and ageing were determined by HPLC- ELSD.Sensory evaluation of flavor and microorganisms were detected after the completion of the brewing.The results showed that main sugars in ice grape juice were glucose and fructose.During the process of fermentation,the glucose content in the ice wine decreased from 180.56 g / L to 53.24 g / L,what's more,the period from 7 to 28 days had highest decline rate. Fructose content in the ice wine decreased from 223.65 g / L to160.54 g / L.And the changes of ethanol concentration were negatively correlated with changes in glucose content,the final alcoholic degree was 11% v / v. During the process of ageing in oak barrel,the glucose content in the ice wine decreased to 47.64 g / L.The fructose content decreased to 151.25 g / L,while final alcoholic degree was 9.8%v / v.Microbial detection and flavor assessment were in line with the standard. The research provided theoretical basis for improving the processing technique and quality of ice wine,at the same time laid a foundation for identification of ice wine.