Abstract: Pseudomonas Fluorescens belongs to Pseudomonas. It was a very common psychrotrophic pathogenic microorganisms in foods. It could produce some cellular enzymes with high thermal stability such as lipases and proteases. After treated at high temperature,these enzymes were still active and continue to decompose the fat and protein in foods. Therefore,recently more and more researchers focus the attention on the rapid and exact detection of Pseudomonas Fluorescens in foods. Some rapid detection methods were discussed in this paper,such as polymerase chain reaction(PCR),realtime fluores-cence quantitative PCR,fluorescence In situ hybridization and flow cytometry,and their advantages and disvantages were compared. At last,the research direction of Pseudomonas Fluorescens was discussed.