重楼叶多糖改善D-半乳糖衰老模型小鼠脾脏免疫功能和抗氧化能力

刘功成; 王作伟; 李亭亭; 杜金莹; 杜小刚; 曹晓涵; 曾宪垠

doi:10.13386/j.issn1002-0306.2015.16.066

重楼叶多糖改善D-半乳糖衰老模型小鼠脾脏免疫功能和抗氧化能力

Effect of polysaccharides from the leaves of Paris polyphylla on immune function and antioxidant capacities of mouse spleen tissue in a D-galactose-induced aging mouse model

摘要

摘要: 采用D-半乳糖注射诱导衰老模型,探讨了重楼叶多糖（PPLPs）对小鼠脾脏免疫功能和抗氧化的影响。分别对小鼠D-半乳糖、D-半乳糖和PPLPs、D-半乳糖和VC以及生理盐水处理,持续42 d。结果显示:与模型组相比,灌胃PPLPs或VC均能显著增加小鼠脾脏指数（p<0.05）,显著上调脾脏免疫相关基因（T-bet、GATA、IFN-γ、IL-2、TNF-α、IL-4和IL-10）m RNA表达水平（p<0.05）,也显著增强了脾脏总超氧化物歧化酶（T-SOD）、铜锌超氧化物歧化酶（Cu Zn-SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性（p<0.05）;同时,灌胃PPLPs或VC显著降低了脾脏丙二醛（MDA）含量（p<0.05）。结果表明,PPLPs可增强脾脏免疫功能并提高小鼠的抗氧化活性。

Abstract: In the present study,the protective effects of polysaccharides from the leaves of Paris polyphylla(PPLPs)against aging in an accelerated aging mice model induced by D-galactose were investigated. A group of2- month- old C57 BL / 6J mice were treated daily with D- galactose, D- galactose combined with PPLPs,D-galactose combined with vitamin C(VC),and control buffer for 42 d. The results showed that compared with the model group, oral administration of D- galactose- induced aging mice with either PPLPs or VCboth significantly increased spleen indices(p<0.05),the m RNA expression levels of immune function related genes(T-bet,GATA,IFN-γ,IL-2,TNF-α,IL-4,and IL-10)(p<0.05) and the total superoxide dismutase(T-SOD),Cu Zn superoxide dismutase(Cu Zn-SOD),glutathione peroxidase(GSH-Px) and catalase(CAT) in spleen tissue were significantly higher in both PPLPs and VCgroup than those in the model group(p<0.05);besides,the malondialdehyde(MDA) concentrations were decreased in PPLPs and VCgroup compared to those in the model group(p<0.05). The results indicated that PPLPs was able to enhance the immune function and improve the antioxidant capacities of mouse spleen tissue.