响应面分析法优化虾蛄盐溶蛋白快速提取工艺

Optimization of salt-soluble protein from oratoria by response surface methodology

摘要: 为了缩短虾蛄盐溶蛋白的提取时间并得到最佳提取条件,在单因素实验的基础上,选出最佳辅助提取方式,并应用响应面法优化虾蛄盐溶蛋白的提取条件,确定最佳提取条件为:磁力搅拌时间为24 min,Na Cl浓度为0.11 mol/L,提取液p H为6.36,料液比为1∶3 g/m L,此时虾蛄盐溶蛋白含量达22.46 mg/g,与预测值(22.71 mg/g)相差0.25 mg/g。

Abstract: To optimize the fast extraction condition for the oratosquilla oratoria salt-soluble protein,the best assisted extraction methods was obtained on the basis of single-factor investigation. The optimize extraction conditions were as follow:extraction solution p H of 6.36,extraction solution concentration of Na Cl was 0.11 mol/L,material to water ratio at 1∶3,and magnetic stirring extraction-duration of 24 min. The salt-soluble protein content could reach 22.46 mg/g,and the theory predicted value(22.71 mg/g) differs by 0.25 mg/g.