秦巴杜仲雄花总黄酮的提取及抗氧化稳定性研究
Extraction of flavone from male flowers of Qinba Eucommia ulmoides and their antioxidant stability
-
摘要: 以秦巴杜仲雄花为原料,采用微波预处理-乙醇回流法,在不同的乙醇浓度、提取温度、提取时间和料液比下,探讨秦巴杜仲雄花总黄酮的提取工艺。通过清除DPPH自由基法测定总黄酮的抗氧化活性,并研究不同条件下总黄酮抗氧化稳定性。结果表明,乙醇浓度为60%,温度为80℃,时间为2.5 h,料液比为1∶60时,杜仲雄花总黄酮得率最高,达到2.983%。温度和金属离子Na+、K+、Ca2+、Mg2+对杜仲雄花总黄酮清除DPPH自由基的能力影响不大;金属离子Cu2+、Zn2+以及p H、光照对抗氧化能力影响较大。Abstract: The extraction process of flavone in male flowers of Qinba Eucommia ulmoides assisted by microwave pretreatment was studied. The flavone extraction from the different ethanol concentration,extraction time,extraction temperature and solid-to-liquid ratio were studied. And the antioxidant stability of total flavonoe was assessed by its capacity to scavenge the DPPH free radical under different treatments. The results showed that the best extraction process condition was:ethanol concentration 60%,abstracting temperature 80 ℃,abstracting time 2.5 h and the ratio of solid to extracting liquid 1∶60(g/m L). The total flavone extraction yield could reach2.983% in the optimum conditions. The temperature and metal ions(Na+,K+,Ca2+,Mg2+) did not produce a great reduction of scavenging capacity. While the Zn2 +,Cu2 +,p H and illumination had a bigger influence to the antioxidation stability of flavone in male flowers of Qinba Eucommia ulmoides.
下载: