巨尾桉叶、皮、芯中单宁提取及其抗氧化性研究

周洲; 龙凤香; 邓黎娟; 韦婷馨; 郑燕菲; 刘雄民

doi:10.13386/j.issn1002-0306.2015.16.018

巨尾桉叶、皮、芯中单宁提取及其抗氧化性研究

Extraction,antioxidant activity of tannins from different parts of Eucalyptus Grandis x E.Urophylla

摘要

摘要: 采用加热回流与超声波辅助提取法分别提取了巨尾桉的树叶、树皮、树芯3部位中的单宁,考察了3部位提取物的抗氧化特性。加热回流法提取巨尾桉树叶、树皮、树芯单宁的得率分别为4.19%、1.86%和0.25%。超声波辅助提取法提取巨尾桉树叶、树皮、树芯单宁的得率分别为3.20%、2.25%和0.19%。巨尾桉3部位的单宁提取物对DPPH·、·OH自由基和H2O2均有较好的清除作用,其中,巨尾桉叶、皮、芯单宁提取物对DPPH·自由基清除率分别相当于VC的91.08%、93.20%、79.40%;对·OH自由基清除率分别相当于VC的181%、125%、132%;对H2O2的清除率分别相当于VC的83.60%、65.46%、78.46%。

Abstract: Conventional refluxing extraction and ultrasound assisted extraction were adopted to extract tannins from leaf,bark and core of Eucalyptus Grandis x E.Urophylla. Moreover the antioxidant activity of tannins extracts also was studied. The yields of tannins from leaf,bark and core by conventional refluxing extraction were 4.19%,1.86% and 0.25% respectively,but that were 3.20%,2.25% and 0.19% respectively by ultrasound assisted extraction. The tannins extracts of Eucalyptus Grandis x E.Urophylla different parts had a strong scavenging activity to DPPH·,·OH free radical and H2O2. The scavenging activity of tannins extracts from leaf,bark and core on DPPH· were the equivalent to VCof 91.08%,93.20% and 79.40%,respectively. And the scavenging activity of 3 tannins extracts on ·OH reached 181%,125% and 132% of VC,on H2O2 equal to83.60%,65.46% and 78.46% VC.