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中国精品科技期刊2020
郑璐, 王兴国, 韩飞, 梁亚静, 第文龙, 陈曦, 李爱科, 管骁. 发芽对糜子酚类化合物及抗氧化活性的影响[J]. 华体会体育, 2015, (16): 124-128. DOI: 10.13386/j.issn1002-0306.2015.16.017
引用本文: 郑璐, 王兴国, 韩飞, 梁亚静, 第文龙, 陈曦, 李爱科, 管骁. 发芽对糜子酚类化合物及抗氧化活性的影响[J]. 华体会体育, 2015, (16): 124-128. DOI: 10.13386/j.issn1002-0306.2015.16.017
ZHENG Lu, WANG Xing-guo, HAN Fei, LIANG Ya-jing, DI Wen-long, CHEN Xi, LI Ai-ke, GUAN Xiao. Effect of germination on phenolics and antioxidant activity of proso millet[J]. Science and Technology of Food Industry, 2015, (16): 124-128. DOI: 10.13386/j.issn1002-0306.2015.16.017
Citation: ZHENG Lu, WANG Xing-guo, HAN Fei, LIANG Ya-jing, DI Wen-long, CHEN Xi, LI Ai-ke, GUAN Xiao. Effect of germination on phenolics and antioxidant activity of proso millet[J]. Science and Technology of Food Industry, 2015, (16): 124-128. DOI: 10.13386/j.issn1002-0306.2015.16.017

发芽对糜子酚类化合物及抗氧化活性的影响

Effect of germination on phenolics and antioxidant activity of proso millet

  • 摘要: 探讨了发芽时间对糜子中酚类化合物含量、存在形式及抗氧化活性的影响。将经过表面灭菌的糜子种子放入25℃,相对湿度95%的人工气候箱中避光发芽6 d,每天取样,测定其中酚、黄酮含量及抗氧化指标(DPPH、ABTS、FRAP、ORAC)的变化。结果显示,从发芽2 d起,酚、黄酮含量及各项抗氧化指标明显增高。发芽6 d后,内糜8号总酚、总黄酮含量分别为发芽前的5.9和1.9倍,西农10-04号则分别为发芽前的4.6和1.9倍,抗氧化能力均显著增强(p<0.05)。此外,发芽4 d后芽中总酚、总黄酮含量及抗氧化能力显著高于(p<0.05)相应种子。总酚、总黄酮与抗氧化能力间均显示出极强的相关性,其中以总黄酮与FRAP相关性最高(r=0.985)。研究表明,发芽是一种有效提高糜子中酚类化合物含量及抗氧化活性的加工方式。 

     

    Abstract: The influence of germination on antioxidant activity,phenolics content and form of proso millet were studied. The changes of phenolics,flavonoids and antioxidant properties(DPPH and ABTS,FRAP,ORAC) of surface sterilized proso millet seeds during germination were determined in artificial climate box for 6 d at 25 ℃and RH 95%. The result showed that the contents of phenolics,flavonoids and antioxidant properties increase obviously from 2 d. Germination after 6 d,the total content of phenolics and flavonoids of NM8 increase 5.9 and1.9 times respectively,and 4.6 and 1.9 times for XN10-04,meanwhile,its antioxidant capacity increase significantly(p <0.05). In addition,total content of phenolics and flavonoids and antioxidant capacity of the sprouts were significantly higher( p < 0. 05) than that of seeds after 4 d. Correlation coefficients between total phenolics content,total flavonoids content and antioxidant activities were positively high,the highest correlation coefficient was found between total flavonoids content and FRAP(r=0.985). This study showed that germination was an effective method to increase the content of phenolic compounds and antioxidant activity of proso millet.

     

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