60Coγ射线复合硫酸二乙酯诱变选育高产新型细菌素Plantaricin 163-1菌株的研究
Screening of High- yeilding Plantaricin 163-1Strains induced by 60Coγ- ray and Diethyl sulfate
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摘要: 为提高Lactobacillus plantarum 163产的新型细菌素Plantaricin 163-1的抑菌相对效价,以Lb.plantarum 163为出发菌株,通过60Coγ射线复合硫酸二乙酯（DES）诱变,以期得到具有稳定遗传性且细菌素高产的菌株。结果表明:60Coγ射线诱变的最佳辐照剂量为0.6 k Gy,获得了抑菌活性提高23.68%的突变株。然后,在35℃下用0.7%硫酸二乙酯复合处理突变菌体25 min,最终得到一株高产突变菌株L150,其相对抑菌效价是原始菌株2.11倍。通过5代传代培养,遗传稳定性良好。Abstract: To obtain high yield and stable strain for the production of plantaricin 163- 1,60Coγ- ray and Diethyl sulfate were employed to induce Lactobacillus plantarum 163 to enhance the production of a novel bacteriocin Plantaricin 163-1.Through the method60Coγ- ray and diethyl sulfate( DES) mutagenesis,the optimum parameters of60Coγ- ray and diethyl sulfate was determined by observing the lethal rate of each methods,which irradiation dose of60Coγ- ray was 0.6 k Gy and DES treatment was 0.7%,treated for 25 min,at 35 ℃.At last,the high- yielding mutant strain L150 was obtained and had stable inheritance. The antibacterial activity of the mutant L150 was 2.11 times comparison with original strain.
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