人工养殖大鲵皮体外消化及其胶原蛋白提取和功能性质研究
Study on the extraction and functional properties of collagens from the skin of Chinese giant salamander and its digestibility in vitro
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摘要: 通过体外消化法测定大鲵皮体外消化率;使用Box-Behnken设计响应面实验,优化大鲵皮胶原蛋白提取工艺;并对胶原蛋白冻干样的功能性质进行研究。结果表明:经胃蛋白酶和胰蛋白酶处理的大鲵皮,其体外消化率高达96.66%;以加酶量、超声时间和提取时间为三因素,胶原蛋白提取率为响应值,利用响应面设计优化大鲵皮胶原蛋白提取率,其最优化参数为加酶量1000 U/m L料液、提取时间33 h以及超声时间12 min,此条件下最优大鲵皮胶原蛋白提取率为71.94%,与模型预测值（73.32%）较一致;在20℃、相对湿度43%和81%条件下,大鲵皮胶原蛋白能长时间保留20%水分;大鲵皮胶原蛋白在0.4 g/100 m L浓度,溶液p H7以及溶液盐离子浓度为1.25 mol/L时有最佳起泡性;当溶液p H4,盐离子浓度为0.25 mol/L时鲵皮胶原蛋白有最佳乳化性。Abstract: The in vitro digestion of the Chinese giant salamander skin was evaluated. The Box- Behnken design response surface test was used to optimize the extraction process of the collagen of the Chinese giant salamander skin.The functional properties of the collagen were also studied.The results showed that: the in vitro digestion of the Chinese giant salamander skin had reached 96.66%.The optimal condition for the extraction were the combination of adding 1000 U / m L of enzyme,33 h of extraction and 12 min of ultrasonic.The yield reached 71.94%,similar with the model predicted value( 73.32%).Under the condition of 20 ℃ and 43% and 81% RH,20% of water could be hold for a long time.The pepsin soluble collagen( PSC) had the best foaming ability when the protein concentration was 0.4 g /100 m L,p H7 and the salt ion concentration was 1.25 mol / L.When p H was 4,salt ion concentration was0.25 mol / L,the salamander skin collagen had the best emulsibility.
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