不同提取方法对红芪多糖体外抗氧化活性的影响研究

寇宁; 李磊强; 李钦; 程卫东

doi:10.13386/j.issn1002-0306.2015.15.013

不同提取方法对红芪多糖体外抗氧化活性的影响研究

Study on anti- oxidant activity of polysaccharides from Hedysarum polybotrys Hand.-Mazz.by different extraction method in vitro

摘要

摘要: 红芪多糖（HPS）是中药红芪中的主要成分。本文分别采用微波辅助、超声辅助及常规热水浸提从红芪中提取HPS,经脱蛋白、冷冻干燥后得到三种方法提取的红芪多糖HPS-M、HPS-C和HPS-H。红外光谱分析表明,HPS-M、HPS-C、HPS-H具有多糖的特征吸收峰;GPC测定结果显示,HPS-M、HPS-C、HPS-H的重均分子量分别为6.29×105、4.56×105、5.13×105,多分散性分别为3.42、3.22、2.31。HPS-H、HPS-M、HPS-C清除羟自由基、超氧自由基、DPPH自由基及还原力测定的体外抗氧化活性实验表明,HPS-M的体外抗氧化活性要显著高于HPS-C、HPS-H,因此,未来可考虑微波辅助法作为红芪多糖提取的首选方法。

Abstract: Polysaccharides were one of the main components of Hedysarum polybotrys Hand.- Mazz( HPS).In this paper,the antioxidant activity of polysaccharides from Hedysarum polybotrys Hand.- Mazz with microwave-assisted extraction,ultrasonic assisted extraction and hot water extraction methods was investigated. HSP- M,HSP- C and HSP- H after deproteinization and vacuum freeze- drying were characterized by FT- IR and measured by GPC.The result indicated that HSP- M,HSP- C and HSP- H had typical absorption peak of polysaccharides,weight average molar mass( MW) were 6.29 × 105,4.56 × 105,5.13 × 105 and polydispersion and gyroradius determined were 3.42,3.22,2.31. Anti- oxidant effect on scavenging DPPH radicals,OH radicals and superoxide radicals and reducing power on HSP- M,HSP- C and HSP- H were measured. The resualts showed that anti-oxidant effect of HSP- M was higher than HSP- C and HSP- H. To get practical methods for the research and manufacture of HPS,microwave- assisted extraction method should be chosen.