Abstract: According to the health food inspection and evaluation of technical specifications, impact of oligosaccharide on defecation and intestinal flora had been researched by detected the mice intestinal propulsive rate,the first time grain black stools,ranked melena grains and weight. There was no significant difference in intestinal propulsive rates between model group of mice and intervention groups. And there were significant differences( p <0.05) on the first grain black stools time, defecation quality and weight between model group and intervention groups. After the xylooligosaccharides intervention, a significant( p < 0. 05)increase in the number of bifidobacteria and lactobacilli,and there was significant difference(p<0.05) compare intervention groups with the control group. According this result,positive effect of defecation promoting and intestinal flora regulation of xylooligosaccharides could be determined.