Abstract: In order to optimize the process of extraction of pectin from blood pomelo peel. Based on the singlefactor experiments,Box-Benhnken design involving four factors as independent variables was used to set up a second order quadratic equation and the applicability of the model for the yield of pectin as function of citric acid concentration,temperature,liquid to material,time. Each variable and their interactions effects on the yield were studied by using Design-Expert software. The results showed that the effect order of four factors on the yield of pectin was as follows:extraction temperature,citric acid concentration,extraction temperature,liquid to material and extraction time. And the optimal extraction conditions of pectin from blood pomelo peel were:citric acid concentration 1.5%,temperature 82℃,liquid to material 32∶1m L·g-1,time 99 min. The yield of pectin could be up to 18.85%,under the optimal extraction condition,the relative error was 0.32% compared to the predictive value,which indicated the feasible model fitted well with the experimental data.